Topic 60

plasmodium malaria falciparum parasite parasites berghei stages mosquito stage blood cycle asexual antimalarial microbiology sporozoite transmission invasion host artemisinin drug erythrocyte erythrocytes sporozoites infection essential erythrocytic gametocyte gametocytes life mosquitoes gondii toxoplasma antimalarials oocyst intraerythrocytic development vaccine drugs role merozoites against malarial apicomplexan protein merozoite circumsporozoite red spp liver rodent strategies egress surface parasitophorous divergent conserved growth vivax proteins hepatocytes membrane antibodies vacuole elimination pf eukaryotes motility rbcs hosts replication forms resistance vitro sexual atovaquone csp target conditional infected protection knowlesi antigens rbc during targeting atypical anopheles vaccines complex apicoplast oocysts intracellular new eukaryotic potent intervention understood invade treatment candidate

139 items. Top items listed below.

Exposure to artemisinin at the trophozoite stage increases sexual conversion rates in the malaria parasite Plasmodium falciparum 60 48

Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytic cells. 60 48 41 3

Gliding motility of Plasmodium merozoites 60 3

Signalome-wide assessment of erythrocyte response to Plasmodium reveals novel targets for host-directed antimalarial intervention 60 3

Erythrocyte CD55 facilitates the internalization of Plasmodium falciparum parasites 60 33 3

Using Plasmodium knowlesi as a model for screening Plasmodium vivax blood-stage malaria vaccine targets reveals new candidates 60 48 12 10

The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei 60 33

Artemisinin-resistant malaria parasites show enhanced transmission to mosquitoes under drug pressure 60 48 12 10

A multipass membrane protein interacts with the cGMP-dependent protein kinase to regulate critical calcium signals in malaria parasites 60 34 3

Lipoic acid biosynthesis is essential for Plasmodium falciparum transmission and influences redox response and carbon metabolism of parasite asexual blood stages 60 48 3

A system-level gene regulatory network model for Plasmodium falciparum 60 9

Single cell sequencing of the small and AT-skewed genome of malaria parasites 60 4

Gene regulation of the avian malaria parasite Plasmodium relictum, during the different stages within the mosquito vector 60 48 9 3

Intrinsic multiplication rate variation and plasticity of human blood stage malaria parasites 60 12 5

Kinetics of Plasmodium midgut invasion in Anopheles mosquitoes 60 48 18

Plasmodium falciparum Replication factor C subunit 1 is involved in genotoxic stress response 60 3

Fluorescent tagging of Plasmodium circumsporozoite protein allows imaging of sporozoite formation but blocks egress from oocysts 60 48 34 8 3

Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum 60

CRISPR/Cas9-engineered inducible gametocyte producer lines as a novel tool for basic and applied research on Plasmodium falciparum malaria transmission stages 60 48 33

Dissection of Plasmodium falciparum developmental stages with multiple imaging methods 60 18 8

Complexome profile of Toxoplasma gondii mitochondria identifies a divergent cytochrome bc1 complex 60 34 3

A dispensable role of mitochondrial fission protein 1 (Fis1) in the erythrocytic development of Plasmodium falciparum 60 34 8 3

Divergent features of the coenzyme Q:cytochrome c oxidoreductase complex in Toxoplasma gondii parasites 60 34 3

Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays 60

Plasmodium falciparum sexual parasites regulate infected erythrocyte permeability 60 3

Host porphobilinogen deaminase deficiency confers malaria resistance in Plasmodium chabaudi but not in Plasmodium falciparum or Plasmodium berghei during intraerythrocytic growth 60 10 3

Novel functional insights from the Plasmodium falciparum sporozoite-specific proteome by probabilistic integration of 26 studies 60 9

A single-cell atlas of Plasmodium falciparum transmission through the mosquito 60 48 9

Co-option of Plasmodium falciparum PP1 for egress from host erythrocytes 60 3

The importance of the immunodominant CD8+ T cell epitope of Plasmodium berghei circumsporozoite protein in parasite- and vaccine-induced protection 146 60 24 10 3

Use of a highly specific kinase inhibitor for rapid, simple and precise synchronization of Plasmodium falciparum and Plasmodium knowlesi asexual stage parasites 60 18

Cyclical Regression Covariates remove the major confounding effect of cyclical developmental gene expression with strain-specific drug response in the malaria parasite Plasmodium falciparum 60 4

Identification and characterisation of a phospholipid scramblase in the malaria parasite Plasmodium falciparum 60 12

Dissection-independent production of a protective whole-sporozoite malaria vaccine 60 48 12

Phosphatidylinositol 3-phosphate and Hsp70 protect Plasmodium falciparum from heat-induced cell death 60 3

Structure of the cysteine-rich domain of Plasmodium falciparum P113 identifies the location of the RH5 binding site 60 8 3

Anopheles gambiae maternal age and parous state control offspring susceptibility to Plasmodium falciparum 60 48 5

Structure-based identification and functional characterization of an essential lipocalin in the malaria parasite Plasmodium falciparum 60 3

Selective expression of variant surface antigens enables Plasmodium falciparum to evade immune clearance in vivo 60 27 10 3

Zonal human hepatocytes are differentially permissive to Plasmodium falciparum malaria parasites 60 48 12

Transgenic pyrimethamine-resistant P. falciparum reveals transmission blocking potency of P218, a novel antifolate 60 48 12

Plasmodium falciparum Atg18 localization to the food vacuole is phosphatidylinositol 3-phosphate-dependent and quinoline-sensitive 60 34 3

A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of Plasmodium berghei during gametogony and parasite transmission. 60 34 3

Antagonistic roles of NOT1 paralogues in the timing of gene expression in Plasmodium falciparum 60 49 3

Th2-like T-follicular helper cells promote functional antibody production during Plasmodium falciparum infection 146 88 60 27 24 10 3

APEX-based proximity labeling in Plasmodium identifies a membrane protein with dual functions during mosquito infection 60 34 3

MRE11 is crucial for malaria transmission and its absence affects expression of interconnected networks of key genes essential for life 60 48 3

The regulatory genome of the malaria vector Anopheles gambiae: integrating chromatin accessibility and gene expression 31 9 3

Plasmodium falciparum population genetic complexity influences transcriptional profile and immune recognition of highly related genotypic clusters 60 5

An exon DNA element modulates heterochromatin spreading in the master regulator for sexual commitment in malaria parasites 60 31 3

Structure of Full Length Plasmodium Myosin A and its light chain PfELC, dual targets against malaria parasite pathogenesis 169 60 7

A druggable oxidative folding pathway in the endoplasmic reticulum of human malaria parasites 60 34 3

Plasmodium berghei K13 Mutations Mediate In Vivo Artemisinin Resistance That is Reversed by Proteasome Inhibition 60 33 12 10

A lipocalin mediates unidirectional haem biomineralization in malaria parasites 60 3

Multiple blood feeding in mosquitoes shortens the Plasmodium falciparum incubation period and increases malaria transmission potential 48 7 5

Plasmodium oocysts respond with dormancy to crowding and nutritional stress 60 48

High-resolution structures of malaria parasite actomyosin and actin filaments 60 7

Modulation of triple artemisinin-based combination therapy pharmacodynamics by Plasmodium falciparum genotype 60 7

Describing the current status of Plasmodium falciparum population structure and drug resistance within mainland Tanzania using molecular inversion probes 60 5

Actomyosin forces and the energetics of red blood cell invasion by the malaria parasite Plasmodium falciparum 60 55 34 8 3

An equivalence of prokaryotic pore forming proteins of Plasmodium triggers cellular dysfunction responsible for malaria pathogenesis 60 3

Atypical molecular basis for drug resistance to mitochondrial function inhibitors in Plasmodium falciparum 60 12

Rapid emergence of clonal interference during malaria parasite cultivation 60 5

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination 146 60 24 10

Structure of an atypical homodimeric actin capping protein from the malaria parasite 60 8 3

System-wide biochemical analysis reveals ozonide antimalarials initially act by disrupting Plasmodium falciparum haemoglobin digestion 60 10 3

Conservation of the Toxoplasma conoid proteome in Plasmodium reveals a cryptic conoid feature that differentiates between blood- and vector-stage zoites 60 48

A screen for Plasmodium falciparum sporozoite surface protein binding to human hepatocyte surface receptors identifies novel host-pathogen interactions 60 12

Phosphorylation Dependent Assembly of a 14-3-3 Mediated Signaling Complex During Red Blood Cell Invasion by Plasmodium falciparum Merozoites 60 34 3

Mapping of safe and early chemo-attenuated live Plasmodium falciparum immunization identifies immune signature of vaccine efficacy 146 60 24 10

Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress 60 34 8 3

Genetic ablation of the mitochondrial ribosome in Plasmodium falciparum sensitizes the human malaria parasite to antimalarial drugs targeting mitochondrial functions 60 34 3

Platelet Derived Growth Factor Receptor β (PDGFRβ) is a Host Receptor for the human malaria parasite adhesin TRAP 60 3

Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea 60 12 5

Common host variation drives malaria parasite fitness in healthy human red cells 60

Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodium falciparum 60 16

Structural ordering of the Plasmodium berghei circumsporozoite protein repeats by inhibitory antibody 3D11 146 60 24 8 3

Last-come, best served? Mosquito biting order and Plasmodium transmission 48 5

A single point mutation in the Plasmodium falciparum ftsh1 metalloprotease confers actinonin resistance. 60 12

Temperature impacts the transmission of malaria parasites by Anopheles gambiae and Anopheles stephensi mosquitoes 48 17 5

A high affinity human monoclonal antibody against Pfs230 binds multiple parasite stages and blocks oocyst formation in mosquitoes 60 24

Evaluation of near infrared spectroscopy for sporozoite detection in mosquitoes infected with wild-strain parasites from asymptomatic gametocyte carriers in Kilifi Kenya 60 48 15

Longitudinal analysis of naturally acquired antibodies to PfEMP1 CIDR domain variants and their association with malaria protection 60 27 10

A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity 146 60 24 8

Blockade of LAG-3 in PD-L1-deficient mice enhances clearance of blood stage malaria independent of humoral responses 88 27 10 3

Orthosteric-allosteric dual inhibitors of PfHT1 as selective anti-malarial agents 60 44 10 8 3

An essential amino acid synchronises malaria parasite development with daily host rhythms 60 12

An Epitope-based Malaria Vaccine Targeting the Junctional Domain of Circumsporozoite Protein 146 24 10

An antigenic diversification threshold for falciparum malaria transmission at high endemicity 60 5

Avid binding by B cells to the Plasmodium circumsporozoite protein repeat suppresses responses to protective subdominant epitopes 146 60 24 10

Plasmodium DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission 60 34 3

Malaria parasites differentially sense environmental elasticity during transmission 60 48 18

Transgenic pyrimethamine resistant Plasmodium berghei as a model for in vivo anti-DHFR drug testing 60 10

The malaria metabolite HMBPP does not trigger erythrocyte terpene production 60 48

A mating-induced reproductive gene promotes Anopheles tolerance to Plasmodium falciparum infection 48 21 5

The transmission-blocking effects antimalarial drugs revisited: mosquito fitness costs and sporontocidal effects of artesunate and sulfadoxine-pyrimethamine 48

Restructured mitochondrial-nuclear interaction in P. falciparum dormancy and persister survival of artemisinin exposure 60 3

Publicly available transcriptomes provide the opportunity for dual RNA-Seq meta analysis in Plasmodium infection 60 9 4

Plasmodium vinckei genomes provide insights into the pan-genome and evolution of rodent malaria parasites 60 40 5

Plasmodium falciparum-specific IgM B cells dominate in children, expand with malaria and produce parasite inhibitory IgM 146 60 27 10 3